Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. cv Reston
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منابع مشابه
Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. cv Reston.
Erucic acid (22:1) was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived (MD) embryo culture system. TAGs accumulating during embryo development exhibited changes in acyl composition similar to those observed in developing zygotic embryos of the same cv, particularly with respect to erucic and eicosenoic acids. However, MD embryos...
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Biosynthesis of very long chain (>C(18)) fatty acids (VLCFAs) and the pathway for their incorporation into acyl lipids was studied in microspore-derived (MD) and zygotic embryos of Brassica napus L. cv Reston. In the presence of [1-(14)C]oleoyl-coenzyme A or [1-(14)C] eicosenoyl-coenzyme A, malonyl-coenzyme A, and reducing equivalents, maximal in vitro elongation activity was expressed in prote...
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The effect of aqueous smoke extract, derived from Tanacetum parthenium, onin vitro plantlet regeneration from rapeseed (Brassica napus L. cv. Topas)microspore-derived embryos (MDEs) was evaluated in this study. Inoculation ofrapeseed MDEs with the smoke-extract dilutions (1:250, 1:500, 1:1000 and 1:2000)for various times (5, 10 and 15 min) significantly enhanced the percentage ofregenerated pla...
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Brassica napus cultivar Westar is non-embryogenic under all standard protocols for induction of microspore embryogenesis; however, the rare embryos produced in Westar microspore cultures, induced with added brassinosteroids, were found to develop into heritably stable embryogenic lines after chromosome doubling. One of the Westar-derived doubled haploid (DH) lines, DH-2, produced up to 30% the ...
متن کاملFatty acid breakdown in developing embryos of Brassica napus L.
Developing Brassica napus embryos are primarily concerned with the accumulation of storage products, namely oil, starch and protein. The presence of fatty acid catabolic pathways in the background of this biosynthetic activity was investigated. Enzymes involved in the process of lipid mobilization, such as malate synthase and isocitrate lyase, are detectable towards the late stages of embryo de...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1991
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.97.1.65